Spectrofluorometric method has been developed for estimation of gatifloxacin and flurbiprofen sodium. (Simultaneous method) The method involves measurement of fluorescence intensity of both drug at wavelength (λmax) of each other (gatifloxacin at 495.96 nm and 312.68 nm, flurbiprofen at 312.68 nm and 495.96 nm). The method has linear response in the range of 40-120 ng/ml and 5-45 ng/ml with co-efficient of correlation, (R2) = 0.9992 for GATI and (R2) =0.9993 for FLURBI The limit of detection and limit of quantification was 12.90 and 39.10 for GATI and 1.5 and 4.7 for FLURBI respectively. Accuracy of the method was performed by % recovery studies. Based on results, proposed spectrofluorometric method was found to be simple, sensitive, accurate and precise for determination of GATI and FLURBI in Ophthalmic dosage form.
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